How scientists study the natural world
Evidence, hypotheses, theories, and laws
Matter, energy, and their transformations
Systems thinking and feedbacks in nature
Links to sustainability and environmental change
By the end of this module, you should be able to:
Describe how scientific knowledge is developed and evaluated
Distinguish hypotheses, theories, and scientific laws
Explain basic properties of matter and energy
Apply the laws of conservation and thermodynamics
Describe systems, feedback loops, and tipping points
Connect physical and biological principles to environmental issues
Peer review
Independent evaluation by other scientists in the field
Replication of results
Findings can be reproduced using the same methods
Transparent methods
Clear description of data, methods, and analyses
Strength of evidence
Multiple lines of evidence support the same conclusion
No absolute proof
Scientific conclusions are always open to revision
Measurement uncertainty
Observations and measurements contain inherent error
Potential for bias
Scientists are human and can be influenced by assumptions
Peer review reduces bias
Independent review helps identify errors and bias
Atoms differ in numbers of subatomic particles
Atomic number determines the element
Mass number
Element
Atoms with a net electric charge
Formed when atoms gain or lose electrons
Loss of electrons → positively charged ion (cation)
Gain of electrons → negatively charged ion (anion)
Ionic form affects solubility, mobility, and biological availability in the environment
Sharing of electrons between atoms to form covalent bonds.
Stronger and much more common than ionic bonds in the molecules of living organisms.
Commonly found in carbon-based organic molecules, such as our DNA and proteins.
Also in inorganic molecules like H2O, CO2, and O2.
One, two, or three pairs of electrons may be shared, making single, double, and triple bonds, respectively. More bonds = stronger; triple bonds are the strongest
Remember the trees in the valley?
Scientists learned how to measure nitrate and nitrite ions in water to determine the concentration of these ions
Determined that lack of trees washed out nitrates from the soil, increasing in concentration in the water runoff.
Acid
A chemical compound that donates H+ ions to solutions.
Aqueous solutions with a pH less than 7 are said to be acidic
Base
A compound that accepts H+ ions and removes them from solution.
Aqueous solutions with a pH greater than 7 are basic or alkaline.
Pure water has a pH very close to 7
If an equal number of these ions are present in a solution the pH will not change as it is said to be buffered.
Organic compounds have at least two carbon atoms and various other elements
Hydrocarbons: contain carbon and hydrogen atoms
Simple carbohydrates: contain carbon, hydrogen, and oxygen
Polymers: simple organic compounds (monomers) chemically bonded together
What are the major types of polymers?
Complex carbohydrates: two or more monomers of simple sugars such as glucose
Proteins: formed by amino acids (that are monomers)
Nucleic acids: such as DNA and RNA that are formed by nucleotides (that are monomers)
Physical changes
Change form or state (e.g., ice to water) without changing chemical identity
Chemical changes
Rearrangement of atoms to form new substances
Chemical equations
Represent reactants, products, and conservation of atoms
Law of conservation of matter
Matter is neither created nor destroyed in physical or chemical changes
Atoms are rearranged, not created or lost
Balanced chemical equations
Ensure the same number of each type of atom on both sides of a reaction
Energy
The ability to do work or transfer heat
First law of thermodynamics
Energy is conserved; it changes form but is not created or destroyed
Second law of thermodynamics
Energy transformations result in lower-quality, less usable energy
Potential energy
Stored energy that has the capacity to do work
Example:
Potential energy can be converted to kinetic energy
Commercial energy
Energy extracted, processed, and sold by humans
Includes:
Most commercial energy currently comes from fossil fuels
Feedback loop
Occurs when a system output feeds back as an input, influencing future system behavior
Positive feedback
Amplifies change, pushing the system further in the same direction
Negative feedback
Dampens change, promoting stability
Example of positive feedback:
Tipping point
A threshold beyond which a system shifts rapidly to a new state
Often triggered by sustained pressure or positive feedbacks
After a tipping point:
Examples:
Negative feedback loop
Counteracts change and promotes system stability
Example: thermostat
Environmental example: aluminum recycling

BIOL 346 An Ecological Perspective | Spring 2026